Abstract
Blood culture is probably the most significant specimen used for the diagnosis of bacterial infections, especially for bloodstream infections. In the present study, we compared the resin-containing BD BACTEC™ Plus-Aerobic (Becton Dickinson), non-charcoal-containing BacT/Alert® SA (bioMérieux), and charcoal-containing BacT/Alert® FA (bioMérieux) blood culture bottles with direct identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 103 bacterial isolates, from clinical blood cultures, representing the most frequent 13 genera and 24 species were examined. Bacteria were extracted from positive blood culture broth by density centrifugation and then subjected to identification by MALDI-TOF MS using two different volumes and chemical treatments. Overall, correct identification by MALDI-TOF MS was obtained for the BD BACTEC™ Plus-Aerobic, BacT/Alert® SA, and BacT/Alert® FA blood culture bottles in 72%, 45.6%, and 23%, respectively, for Gram-negative bacteria in 86.6%, 69.2%, and 47.1%, respectively, and for Gram-positive bacteria in 60.0%, 28.8%, and 5.4%, respectively. The lack of identification was observed mainly with viridans streptococci. Depending on the blood culture bottles used in routine diagnostic procedures and the protocol for bacterial preparation, the applied MALDI-TOF MS represents an efficient and rapid method for direct bacterial identification.
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References
Bizzini A, Durussel C, Bille J, Greub G, Prod’hom G (2010) Performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of bacterial strains routinely isolated in a clinical microbiology laboratory. J Clin Microbiol 48(5):1549–1554
Ferroni A, Suarez S, Beretti JL, Dauphin B, Bille E, Meyer J et al (2010) Real-time identification of bacteria and Candida species in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48(5):1542–1548
Christner M, Rohde H, Wolters M, Sobottka I, Wegscheider K, Aepfelbacher M (2010) Rapid identification of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of flight mass spectrometry fingerprinting. J Clin Microbiol 48(5):1584–1591
Ferreira L, Sánchez-Juanes F, Porras-Guerra I, García-García MI, García-Sánchez JE, González-Buitrago JM et al (2011) Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Clin Microbiol Infect 17(4):546–551
Moussaoui W, Jaulhac B, Hoffmann A-M, Ludes B, Kostrzewa M, Riegel P et al (2010) Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identifies 90% of bacteria directly from blood culture vials. Clin Microbiol Infect 16:1631–1638
La Scola B, Raoult D (2009) Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PLoS One 4(11):e8041
Prod’hom G, Bizzini A, Durussel C, Bille J, Greub G (2010) Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets. J Clin Microbiol 48(4):1481–1483
Stevenson LG, Drake SK, Murray PR (2010) Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48(2):444–447
Szabados F, Michels M, Kaase M, Gatermann S (2011) The sensitivity of direct identification from positive BacT/ALERT™ (bioMérieux) blood culture bottles by matrix-assisted laser desorption ionization time-of-flight mass spectrometry is low. Clin Microbiol Infect 17(2):192–195
Romero-Gómez MP, Mingorance J (2011) The effect of the blood culture bottle type in the rate of direct identification from positive cultures by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry. J Infect 62(3):251–253
Cherkaoui A, Hibbs J, Emonet S, Tangomo M, Girard M, Francois P et al (2010) Comparison of two matrix-assisted laser desorption ionization-time of flight mass spectrometry methods with conventional phenotypic identification for routine identification of bacteria to the species level. J Clin Microbiol 48(4):1169–1175
Acknowledgments
The authors acknowledge Elke Jurasch, Ellen Heimisch, Maureen de la Rambelje, and Steffi Scholles for their expert technical advice, and André Frontzek for the 16S rDNA sequencing.
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Vera Schmidt designed and performed the experiments, analyzed the data, and prepared the tables; Anja Jarosch assisted in the MALDI-TOF MS analyses; Polina März and Charline Sander assisted in the performance of the experiments; Vladimir Vacata performed the statistical analyses; and Wiltrud Kalka-Moll designed the experiments, analyzed the data, and wrote the manuscript.
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Schmidt, V., Jarosch, A., März, P. et al. Rapid identification of bacteria in positive blood culture by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Eur J Clin Microbiol Infect Dis 31, 311–317 (2012). https://doi.org/10.1007/s10096-011-1312-0
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DOI: https://doi.org/10.1007/s10096-011-1312-0