RT Journal Article
SR Electronic
T1 What is proteomics?
JF Archives of disease in childhood - Education & practice edition
JO Arch Dis Child Educ Pract Ed
FD BMJ Publishing Group Ltd and Royal College of Paediatrics and Child Health
SP edpract-2019-317434
DO 10.1136/archdischild-2019-317434
A1 Andrew James McArdle
A1 Stephanie Menikou
YR 2020
UL http://ep.bmj.com/content/early/2020/04/02/archdischild-2019-317434.abstract
AB Proteomics is the study of a large number of proteins in biological systems. We aim to introduce the complex field to paediatricians and present some recent examples of applications to paediatric problems. Various approaches have been used to study proteomes. The current mainstay is tandem mass spectrometry of enzymatically digested proteins (‘bottom-up proteomics’), and we describe the experimental and computational approach further. Proteomics can offer advantages over transcriptomics by giving direct information about proteins rather than RNA; however, typically data are obtained at lower depth and the confident identification of mass spectra can be challenging. Proteomics frequently complements transcriptomics and other -omics. Used effectively, proteomics offers promise to help answer important clinical and biological questions.